Polymerase chain reaction

Polymerase chain reaction

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Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, who was an employee of the Cetus Corporation, and also the winner of Nobel Prize in Chemistry in 1993, it is an easy, cheap, and reliable way to repeatedly replicate a focused segment of DNA, a concept which is applicable to numerous fields in modern biology and related sciences. PCR is probably the most widely used technique in molecular biology. This technique is used in biomedical research, criminal forensics, and molecular archaeology.

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